Review



pre-plated mdckii-mdr1 cells corning hts transwell-96  (Corning Life Sciences)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Corning Life Sciences pre-plated mdckii-mdr1 cells corning hts transwell-96
    Pre Plated Mdckii Mdr1 Cells Corning Hts Transwell 96, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/corning+transwell+plates/mdck+cells/us12358928-3416-27-30
    Average 90 stars, based on 1 article reviews
    pre-plated mdckii-mdr1 cells corning hts transwell-96 - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: Beyond epithelial-to-mesenchymal transition: common suppression of differentiation programs underlies epithelial barrier dysfunction in mild, moderate and severe asthma
    Article Snippet: Normal human bronchial epithelial cells and diseased human bronchial epithelial cells (asthma) were purchased from Lonza and grown either in submerged conditions, or in fully differentiated organotypic conditions (>3 weeks of culture at air-liquid interface in specialized differentiation media in 0.4 μm Corning Transwell plates, following Lonza's protocol).

    Modification:

    Article Title: Waterpipe tobacco smoke toxicity: the impact of waterpipe size
    Article Snippet: .. Cell maintenance and exposure Rat alveolar type II (L2) cells (ATCC#: CCL-149) were maintained and exposed as per Bernd et al . 20 Briefly, air-liquid interface (ALI) cultures included 1×10 6 cells seeded apically in 12-well Corning Transwell plates with 1.5 mL basolateral low glucose media (LGM: (1 g/L) Dulbecco’s Modified Eagle’s Media, 10% Fetal Bovine Serum, 1× Antibiotic-Antimycotic (Gibco #15240062)). .. Cell maintenance and exposure Rat alveolar type II (L2) cells (ATCC#: CCL-149) were maintained and exposed as per Bernd et al . 20 Briefly, air-liquid interface (ALI) cultures included 1×10 6 cells seeded apically in 12-well Corning Transwell plates with 1.5 mL basolateral low glucose media (LGM: (1 g/L) Dulbecco’s Modified Eagle’s Media, 10% Fetal Bovine Serum, 1× Antibiotic-Antimycotic (Gibco #15240062)).

    Article Title: Waterpipe tobacco smoke toxicity: the impact of waterpipe size
    Article Snippet: .. Rat alveolar type II (L2) cells (ATCC#: CCL-149) were maintained and exposed as per Bernd et al . 20 Briefly, air-liquid interface (ALI) cultures included 1×10 6 cells seeded apically in 12-well Corning Transwell plates with 1.5 mL basolateral low glucose media (LGM: (1 g/L) Dulbecco’s Modified Eagle’s Media, 10% Fetal Bovine Serum, 1× Antibiotic-Antimycotic (Gibco #15240062)). ..

    Incubation:

    Article Title: METTL1/FOXM1 promotes lung adenocarcinoma progression and gefitinib resistance by inhibiting PTPN13 expression.
    Article Snippet: .. After 6- wells' Corning Transwell plates (the diameter of aperture: 8 μm) were pretreated with the mixture of serum- free medium and Matrigel (ratio: 8:1), 5 × 105 cells were incubated on the up- nacelle for 48 h. Next, cells stained by crystal violet were counted and compared for statistical significance. ..

    Article Title: METTL1/FOXM1 promotes lung adenocarcinoma progression and gefitinib resistance by inhibiting PTPN13 expression
    Article Snippet: .. After 6‐wells' Corning Transwell plates (the diameter of aperture: 8 μm) were pretreated with the mixture of serum‐free medium and Matrigel (ratio: 8:1), 5 × 10 5 cells were incubated on the up‐nacelle for 48 h. Next, cells stained by crystal violet were counted and compared for statistical significance. ..

    Staining:

    Article Title: METTL1/FOXM1 promotes lung adenocarcinoma progression and gefitinib resistance by inhibiting PTPN13 expression.
    Article Snippet: .. After 6- wells' Corning Transwell plates (the diameter of aperture: 8 μm) were pretreated with the mixture of serum- free medium and Matrigel (ratio: 8:1), 5 × 105 cells were incubated on the up- nacelle for 48 h. Next, cells stained by crystal violet were counted and compared for statistical significance. ..

    Article Title: METTL1/FOXM1 promotes lung adenocarcinoma progression and gefitinib resistance by inhibiting PTPN13 expression
    Article Snippet: .. After 6‐wells' Corning Transwell plates (the diameter of aperture: 8 μm) were pretreated with the mixture of serum‐free medium and Matrigel (ratio: 8:1), 5 × 10 5 cells were incubated on the up‐nacelle for 48 h. Next, cells stained by crystal violet were counted and compared for statistical significance. ..

    Cell Culture:

    Article Title: Pyrazolamide derivatives inhibit α-Synuclein aggregation, disaggregate preformed fibers, and reduce inclusion formation in neuron cells.
    Article Snippet: α-Syn fibers, the primary cause and central element of Lewy bodies (LB), play a pivotal role in the development of Parkinson’s disease (PD).. This research aims to identify more potent inhibitors of α-Syn aggregation.. A series of N-aryl-3-aryl-pyrazole-5-carboxamide derivatives were designed and synthesized for this purpose.

    Positron Emission Tomography:

    Article Title: Novel 4-Aryl-4H-chromene derivative displayed excellent in vivo anti-glioblastoma efficacy as the microtubule-targeting agent.
    Article Snippet: In this study, a series of novel 4-Aryl-4H-chromene derivatives (D1-D31) were designed and synthesized by integrating quinoline heterocycle to crolibulin template molecule based on the strategy of molecular hybridization.. One of these compounds D19 displayed positive antiproliferative activity against U87 cancer cell line (IC50 = 0.90 ± 0.03 μM).. Compound D19 was verified as the microtubule-targeting agent through downregulating tubulin related genes of U87 cells, destroying the cytoskeleton of tubulins and interacting with the colchicine-binding site to inhibit the polymerization of tubulins by transcriptome analysis, immune-fluorescence staining, microtubule dynamics and EBI competition assays as well as molecular docking simulations.



    Similar Products

    pre-plated mdckii-mdr1 cells corning hts transwell-96  (Corning Life Sciences)
    90
    Corning Life Sciences pre-plated mdckii-mdr1 cells corning hts transwell-96
    Pre Plated Mdckii Mdr1 Cells Corning Hts Transwell 96, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/corning+transwell+plates/mdck+cells/us12358928-3416-27-30
    Average 90 stars, based on 1 article reviews
    pre-plated mdckii-mdr1 cells corning hts transwell-96 - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    matrix-coated corning 96-well transwell® plates  (Corning Life Sciences)
    90
    Corning Life Sciences matrix-coated corning 96-well transwell® plates
    Matrix Coated Corning 96 Well Transwell® Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/corning+transwell+plates/matrix+coated+corning+96+well+transwell++plates/pm40645586-67-23-19
    Average 90 stars, based on 1 article reviews
    matrix-coated corning 96-well transwell® plates - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    transwell plate corning 3462  (Corning Life Sciences)
    90
    Corning Life Sciences transwell plate corning 3462
    Transwell Plate Corning 3462, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/corning+transwell+plates/96+well+plates/pmc12279891-117-24-26
    Average 90 stars, based on 1 article reviews
    transwell plate corning 3462 - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    transwell plate corning  (Corning Life Sciences)
    90
    Corning Life Sciences transwell plate corning
    a Viscosity of rat intestinal mucus treated with or without GlcN (50 mg/mL for 30 min) was examined by rheometer. b The tight junctions between intestinal epithelial cells treated with GlcN (250 mg/kg for 20 min) were detected by TEM. Scare bar, 1 μm. The image only represents the results of one biological sample. c Transcellular transport rate of capecitabine in Caco-2 cells treated with the indicated drugs was examined by <t>transwell</t> model. n = 3 biologically independent samples. d Schematic of validating the interaction between GlcN and P-gp through cell uptake and gene knockout. Created in BioRender. Qinghua, W. (2025) https://BioRender.com/c20pkwq e , f Accumulation ( e ) and efflux ( f ) of Rh123 in Caco-2 cells treated with or without GlcN at the indicated concentrations were determined by a multimode reader (Ex = 485 nm, Em = 528 nm). n = 6 biologically independent samples. g Expression of P-gp in the indicated Caco-2 cells was determined by immunoblot. h Cellular uptake of Rh123 in control and P-gp silenced Caco-2 cells treated with or without GlcN at the indicated concentrations was determined (Ex = 485 nm, Em = 528 nm). n = 6 biologically independent samples (After-silencing, p- value for Verapamil = 0.3034, 1 mM = 0.9996, 10 mM = 0.7150, 15 mM = 0.9993). i , k Pharmacokinetic behavior of digoxin in wild-type (wt) FVB mice ( i ) and abcb1 (-/-) mice ( k ) in the presence or absence of GlcN (500 mg/kg). n = 6 biologically independent mice. j Schematic of the abcb1 gene knockout process in mice. Created in BioRender. Qinghua, W. (2025) https://BioRender.com/c20pkwq . Data are means ± SEM and p- values were calculated using one-way ANOVA in c − h . ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS represent not significant.
    Transwell Plate Corning, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/corning+transwell+plates/96+well+plates/pmc12222954-243-11-13
    Average 90 stars, based on 1 article reviews
    transwell plate corning - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    24-well transwell plate corning, ny  (Corning Life Sciences)
    90
    Corning Life Sciences 24-well transwell plate corning, ny
    a Viscosity of rat intestinal mucus treated with or without GlcN (50 mg/mL for 30 min) was examined by rheometer. b The tight junctions between intestinal epithelial cells treated with GlcN (250 mg/kg for 20 min) were detected by TEM. Scare bar, 1 μm. The image only represents the results of one biological sample. c Transcellular transport rate of capecitabine in Caco-2 cells treated with the indicated drugs was examined by <t>transwell</t> model. n = 3 biologically independent samples. d Schematic of validating the interaction between GlcN and P-gp through cell uptake and gene knockout. Created in BioRender. Qinghua, W. (2025) https://BioRender.com/c20pkwq e , f Accumulation ( e ) and efflux ( f ) of Rh123 in Caco-2 cells treated with or without GlcN at the indicated concentrations were determined by a multimode reader (Ex = 485 nm, Em = 528 nm). n = 6 biologically independent samples. g Expression of P-gp in the indicated Caco-2 cells was determined by immunoblot. h Cellular uptake of Rh123 in control and P-gp silenced Caco-2 cells treated with or without GlcN at the indicated concentrations was determined (Ex = 485 nm, Em = 528 nm). n = 6 biologically independent samples (After-silencing, p- value for Verapamil = 0.3034, 1 mM = 0.9996, 10 mM = 0.7150, 15 mM = 0.9993). i , k Pharmacokinetic behavior of digoxin in wild-type (wt) FVB mice ( i ) and abcb1 (-/-) mice ( k ) in the presence or absence of GlcN (500 mg/kg). n = 6 biologically independent mice. j Schematic of the abcb1 gene knockout process in mice. Created in BioRender. Qinghua, W. (2025) https://BioRender.com/c20pkwq . Data are means ± SEM and p- values were calculated using one-way ANOVA in c − h . ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS represent not significant.
    24 Well Transwell Plate Corning, Ny, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/corning+transwell+plates/96+well+plates/pmc12177566-62-1-4
    Average 90 stars, based on 1 article reviews
    24-well transwell plate corning, ny - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    96-well transwell plate corning 3392  (Corning Life Sciences)
    90
    Corning Life Sciences 96-well transwell plate corning 3392
    a Viscosity of rat intestinal mucus treated with or without GlcN (50 mg/mL for 30 min) was examined by rheometer. b The tight junctions between intestinal epithelial cells treated with GlcN (250 mg/kg for 20 min) were detected by TEM. Scare bar, 1 μm. The image only represents the results of one biological sample. c Transcellular transport rate of capecitabine in Caco-2 cells treated with the indicated drugs was examined by <t>transwell</t> model. n = 3 biologically independent samples. d Schematic of validating the interaction between GlcN and P-gp through cell uptake and gene knockout. Created in BioRender. Qinghua, W. (2025) https://BioRender.com/c20pkwq e , f Accumulation ( e ) and efflux ( f ) of Rh123 in Caco-2 cells treated with or without GlcN at the indicated concentrations were determined by a multimode reader (Ex = 485 nm, Em = 528 nm). n = 6 biologically independent samples. g Expression of P-gp in the indicated Caco-2 cells was determined by immunoblot. h Cellular uptake of Rh123 in control and P-gp silenced Caco-2 cells treated with or without GlcN at the indicated concentrations was determined (Ex = 485 nm, Em = 528 nm). n = 6 biologically independent samples (After-silencing, p- value for Verapamil = 0.3034, 1 mM = 0.9996, 10 mM = 0.7150, 15 mM = 0.9993). i , k Pharmacokinetic behavior of digoxin in wild-type (wt) FVB mice ( i ) and abcb1 (-/-) mice ( k ) in the presence or absence of GlcN (500 mg/kg). n = 6 biologically independent mice. j Schematic of the abcb1 gene knockout process in mice. Created in BioRender. Qinghua, W. (2025) https://BioRender.com/c20pkwq . Data are means ± SEM and p- values were calculated using one-way ANOVA in c − h . ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS represent not significant.
    96 Well Transwell Plate Corning 3392, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/corning+transwell+plates/96+well+plates/pm40586821-95-5-8
    Average 90 stars, based on 1 article reviews
    96-well transwell plate corning 3392 - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    polycarbonate transwell inserts corning tm transwell® 12-well plate  (Corning Life Sciences)
    90
    Corning Life Sciences polycarbonate transwell inserts corning tm transwell® 12-well plate
    a Viscosity of rat intestinal mucus treated with or without GlcN (50 mg/mL for 30 min) was examined by rheometer. b The tight junctions between intestinal epithelial cells treated with GlcN (250 mg/kg for 20 min) were detected by TEM. Scare bar, 1 μm. The image only represents the results of one biological sample. c Transcellular transport rate of capecitabine in Caco-2 cells treated with the indicated drugs was examined by <t>transwell</t> model. n = 3 biologically independent samples. d Schematic of validating the interaction between GlcN and P-gp through cell uptake and gene knockout. Created in BioRender. Qinghua, W. (2025) https://BioRender.com/c20pkwq e , f Accumulation ( e ) and efflux ( f ) of Rh123 in Caco-2 cells treated with or without GlcN at the indicated concentrations were determined by a multimode reader (Ex = 485 nm, Em = 528 nm). n = 6 biologically independent samples. g Expression of P-gp in the indicated Caco-2 cells was determined by immunoblot. h Cellular uptake of Rh123 in control and P-gp silenced Caco-2 cells treated with or without GlcN at the indicated concentrations was determined (Ex = 485 nm, Em = 528 nm). n = 6 biologically independent samples (After-silencing, p- value for Verapamil = 0.3034, 1 mM = 0.9996, 10 mM = 0.7150, 15 mM = 0.9993). i , k Pharmacokinetic behavior of digoxin in wild-type (wt) FVB mice ( i ) and abcb1 (-/-) mice ( k ) in the presence or absence of GlcN (500 mg/kg). n = 6 biologically independent mice. j Schematic of the abcb1 gene knockout process in mice. Created in BioRender. Qinghua, W. (2025) https://BioRender.com/c20pkwq . Data are means ± SEM and p- values were calculated using one-way ANOVA in c − h . ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS represent not significant.
    Polycarbonate Transwell Inserts Corning Tm Transwell® 12 Well Plate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/corning+transwell+plates/polycarbonate+transwell+inserts+corning+tm+transwell++12+well+plate/pmc12163080-375-19-20
    Average 90 stars, based on 1 article reviews
    polycarbonate transwell inserts corning tm transwell® 12-well plate - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    12-well transwell plates corning, catalog no. 3401  (Corning Life Sciences)
    90
    Corning Life Sciences 12-well transwell plates corning, catalog no. 3401
    a Viscosity of rat intestinal mucus treated with or without GlcN (50 mg/mL for 30 min) was examined by rheometer. b The tight junctions between intestinal epithelial cells treated with GlcN (250 mg/kg for 20 min) were detected by TEM. Scare bar, 1 μm. The image only represents the results of one biological sample. c Transcellular transport rate of capecitabine in Caco-2 cells treated with the indicated drugs was examined by <t>transwell</t> model. n = 3 biologically independent samples. d Schematic of validating the interaction between GlcN and P-gp through cell uptake and gene knockout. Created in BioRender. Qinghua, W. (2025) https://BioRender.com/c20pkwq e , f Accumulation ( e ) and efflux ( f ) of Rh123 in Caco-2 cells treated with or without GlcN at the indicated concentrations were determined by a multimode reader (Ex = 485 nm, Em = 528 nm). n = 6 biologically independent samples. g Expression of P-gp in the indicated Caco-2 cells was determined by immunoblot. h Cellular uptake of Rh123 in control and P-gp silenced Caco-2 cells treated with or without GlcN at the indicated concentrations was determined (Ex = 485 nm, Em = 528 nm). n = 6 biologically independent samples (After-silencing, p- value for Verapamil = 0.3034, 1 mM = 0.9996, 10 mM = 0.7150, 15 mM = 0.9993). i , k Pharmacokinetic behavior of digoxin in wild-type (wt) FVB mice ( i ) and abcb1 (-/-) mice ( k ) in the presence or absence of GlcN (500 mg/kg). n = 6 biologically independent mice. j Schematic of the abcb1 gene knockout process in mice. Created in BioRender. Qinghua, W. (2025) https://BioRender.com/c20pkwq . Data are means ± SEM and p- values were calculated using one-way ANOVA in c − h . ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS represent not significant.
    12 Well Transwell Plates Corning, Catalog No. 3401, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/corning+transwell+plates/96+well+plates/pm40464080-38-6-9
    Average 90 stars, based on 1 article reviews
    12-well transwell plates corning, catalog no. 3401 - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    8-micron filtration chamber of the corning transwell 24-well plate  (Corning Life Sciences)
    90
    Corning Life Sciences 8-micron filtration chamber of the corning transwell 24-well plate
    a Viscosity of rat intestinal mucus treated with or without GlcN (50 mg/mL for 30 min) was examined by rheometer. b The tight junctions between intestinal epithelial cells treated with GlcN (250 mg/kg for 20 min) were detected by TEM. Scare bar, 1 μm. The image only represents the results of one biological sample. c Transcellular transport rate of capecitabine in Caco-2 cells treated with the indicated drugs was examined by <t>transwell</t> model. n = 3 biologically independent samples. d Schematic of validating the interaction between GlcN and P-gp through cell uptake and gene knockout. Created in BioRender. Qinghua, W. (2025) https://BioRender.com/c20pkwq e , f Accumulation ( e ) and efflux ( f ) of Rh123 in Caco-2 cells treated with or without GlcN at the indicated concentrations were determined by a multimode reader (Ex = 485 nm, Em = 528 nm). n = 6 biologically independent samples. g Expression of P-gp in the indicated Caco-2 cells was determined by immunoblot. h Cellular uptake of Rh123 in control and P-gp silenced Caco-2 cells treated with or without GlcN at the indicated concentrations was determined (Ex = 485 nm, Em = 528 nm). n = 6 biologically independent samples (After-silencing, p- value for Verapamil = 0.3034, 1 mM = 0.9996, 10 mM = 0.7150, 15 mM = 0.9993). i , k Pharmacokinetic behavior of digoxin in wild-type (wt) FVB mice ( i ) and abcb1 (-/-) mice ( k ) in the presence or absence of GlcN (500 mg/kg). n = 6 biologically independent mice. j Schematic of the abcb1 gene knockout process in mice. Created in BioRender. Qinghua, W. (2025) https://BioRender.com/c20pkwq . Data are means ± SEM and p- values were calculated using one-way ANOVA in c − h . ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS represent not significant.
    8 Micron Filtration Chamber Of The Corning Transwell 24 Well Plate, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/corning+transwell+plates/8+micron+filtration+chamber+of+the+corning+transwell+24+well+plate/pm40472404-179-15-20
    Average 90 stars, based on 1 article reviews
    8-micron filtration chamber of the corning transwell 24-well plate - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    a Viscosity of rat intestinal mucus treated with or without GlcN (50 mg/mL for 30 min) was examined by rheometer. b The tight junctions between intestinal epithelial cells treated with GlcN (250 mg/kg for 20 min) were detected by TEM. Scare bar, 1 μm. The image only represents the results of one biological sample. c Transcellular transport rate of capecitabine in Caco-2 cells treated with the indicated drugs was examined by transwell model. n = 3 biologically independent samples. d Schematic of validating the interaction between GlcN and P-gp through cell uptake and gene knockout. Created in BioRender. Qinghua, W. (2025) https://BioRender.com/c20pkwq e , f Accumulation ( e ) and efflux ( f ) of Rh123 in Caco-2 cells treated with or without GlcN at the indicated concentrations were determined by a multimode reader (Ex = 485 nm, Em = 528 nm). n = 6 biologically independent samples. g Expression of P-gp in the indicated Caco-2 cells was determined by immunoblot. h Cellular uptake of Rh123 in control and P-gp silenced Caco-2 cells treated with or without GlcN at the indicated concentrations was determined (Ex = 485 nm, Em = 528 nm). n = 6 biologically independent samples (After-silencing, p- value for Verapamil = 0.3034, 1 mM = 0.9996, 10 mM = 0.7150, 15 mM = 0.9993). i , k Pharmacokinetic behavior of digoxin in wild-type (wt) FVB mice ( i ) and abcb1 (-/-) mice ( k ) in the presence or absence of GlcN (500 mg/kg). n = 6 biologically independent mice. j Schematic of the abcb1 gene knockout process in mice. Created in BioRender. Qinghua, W. (2025) https://BioRender.com/c20pkwq . Data are means ± SEM and p- values were calculated using one-way ANOVA in c − h . ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS represent not significant.

    Journal: Nature Communications

    Article Title: Glucosamine activates intestinal P-glycoprotein inhibiting drug absorption

    doi: 10.1038/s41467-025-61437-2

    Figure Lengend Snippet: a Viscosity of rat intestinal mucus treated with or without GlcN (50 mg/mL for 30 min) was examined by rheometer. b The tight junctions between intestinal epithelial cells treated with GlcN (250 mg/kg for 20 min) were detected by TEM. Scare bar, 1 μm. The image only represents the results of one biological sample. c Transcellular transport rate of capecitabine in Caco-2 cells treated with the indicated drugs was examined by transwell model. n = 3 biologically independent samples. d Schematic of validating the interaction between GlcN and P-gp through cell uptake and gene knockout. Created in BioRender. Qinghua, W. (2025) https://BioRender.com/c20pkwq e , f Accumulation ( e ) and efflux ( f ) of Rh123 in Caco-2 cells treated with or without GlcN at the indicated concentrations were determined by a multimode reader (Ex = 485 nm, Em = 528 nm). n = 6 biologically independent samples. g Expression of P-gp in the indicated Caco-2 cells was determined by immunoblot. h Cellular uptake of Rh123 in control and P-gp silenced Caco-2 cells treated with or without GlcN at the indicated concentrations was determined (Ex = 485 nm, Em = 528 nm). n = 6 biologically independent samples (After-silencing, p- value for Verapamil = 0.3034, 1 mM = 0.9996, 10 mM = 0.7150, 15 mM = 0.9993). i , k Pharmacokinetic behavior of digoxin in wild-type (wt) FVB mice ( i ) and abcb1 (-/-) mice ( k ) in the presence or absence of GlcN (500 mg/kg). n = 6 biologically independent mice. j Schematic of the abcb1 gene knockout process in mice. Created in BioRender. Qinghua, W. (2025) https://BioRender.com/c20pkwq . Data are means ± SEM and p- values were calculated using one-way ANOVA in c − h . ** p < 0.01, *** p < 0.001, **** p < 0.0001, NS represent not significant.

    Article Snippet: Caco-2 cells (6 × 10 5 cells/well) were seeded in a transwell plate (Corning) for 21 days until the TEER was above 600 Ω·cm 2 .

    Techniques: Viscosity, Gene Knockout, Expressing, Western Blot, Control